Interpretation of the clinical guideline for point-of-care ultrasonography in the neonatal intensive care unit in the United States / 中国当代儿科杂志

In December 2022, the American Academy of Pediatrics released a clinical guideline for point-of-care ultrasonography (POCUS) in the neonatal intensive care unit (NICU). The guideline outlined the development and current status of POCUS in the NICU, and summarized the key elements and implementation guidelines for successful implementation of POCUS in the NICU. This article provides an overview of the key points of the clinical guideline and analyzes the current status of POCUS in China, providing a reference for the implementation of POCUS in neonatal care in China.

Liquid chromatography-mass spectrometry for quantification of the anti-chemotherapeutic antibody in rat plasma / 国际药学研究杂志

Objective To establish a sensitive, accurate and reliable LC-MS method to quantify the monoclonal antibody in rat blood and preclinical pharmacokinetics study. Methods The method of selective reaction monitoring (SRM) was established by using on-the-fly orthogonal array optimization (OAO) with anti-chemotherapeutic antibody-06(ACA-06)as a model drug, and the pharmacokinetics of ACA-06 in rats was studied. The optimal signature peptide selection and mass spectrometry conditions were optimized by single LC-MS run. Two highly specific and sensitive peptides, FSGVPDR and VNSAAFPAPIEK, were identified as the signature peptides of quantification based on the OAO optimization result, which were located in the ACA-06 light chain and heavy chain complementary determining region (CDR) respectively. Results The method had good linearity in the range of 12.9~32 250 ng/ml (r20.9819-0.9946). The lower limit of quantification was 12.9 ng/ml. The intra-day precision (RSD) was less than 11.5%, the inter-day RSD was less than 6%, and the accuracy was between -4.0% and 4.3%. Conclusion In this study, LC/SRM-MS method is established for the determination of ACA-06 in rat plasma. The method is specific, accurate and sensitive and enables an accurate pharmacokinetics investigation of ACA-06 in rat plasma.

Effect of Pirin on the proliferation and self-renew of glioma stem cell / 国际药学研究杂志

Objective To study the effect of Pirin(an iron-binding nuclear protein)on the proliferation and self-renewal of glioma stem cell(GSC)so as to provide a potential therapy target for malignant glioma.Methods PLKO.1-shPirin lentiviral plasmids were constructed to stably knock down Pirin in GSC by using lentivirus infection system.The interference efficiency of Pirin short hair?pin RNA(shRNA)was detected by Western blotting. The capacity of GSC was examined by the assessment of cell viability. Tumor sphere formation assay was used to detect the effect of Pirin on GSC self-renewal capacity. Results Pirin was highly expressed in GSC.Consistently,the protein level of Pirin in the conditioned medium from GSC was much higher than that from the corresponding non-stem tumor cell(NSTC).Gene-sequencing analysis demonstrated that PLKO.1-shPirin lentiviral plasmids were successfully con?structed.Pirin shRNA transfection significantly inhibited the expression of Pirin in GSC and suppressed the cell viability and ability of tumorsphere formation.Conclusion Knocking down Pirin significantly inhibites the cell proliferation and self-renewal of GSC.